Application

Anti- Human IgM was used in an ELISA assay to determine the concentration of IgM secreted by CH12 B lymphoma cells stimulated by LPS.

Goat anti-human IgM (mu-chain specific)-peroxidase antibody can be used: for dot blot (1:100,000) and immunohistochemistry (1:200 using formalin-fixed, paraffin-embedded sections) applications.in immunoblottingsandwich immunoassay

Biochem/physiol Actions

IgM plays a key role in engulfing of the apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

B cells predominantly secrete IgM antibodies as pentamers, but may also secrete them as hexamers which are capable of activating the complement cascade more efficiently. Goat anti-human IgM (mu-chain specific)-peroxidase antibody is specific for human IgM and does not bind to other human Igs. Furthermore, the antibody does not react with mouse and rat IgG. Hence, the antibody conjugate yields reduced background with mouse or rat samples.

Immunogen

human IgM

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).